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Célestine Brunois

Célestine Brunois

PhD in Biomedical Sciences

Creative problem-solving
Strategic thinking
Communication and diplomatic
Teamwork
Neuroscience
29 years old
Driving License
brunoiscelestine@gmail.com +32 498 43 28 48
15 rue des Juifs, 6464 Bourlers, Belgium
I am strongly motivated and inspired to participate to research in Neuroscience
For more details, check my online resume : www.doyoubuzz/celestine-brunois-cv
Experience

Junior Biologist

OncoDNA
Since October 2019
  • Deliver reports from patients analyses to customers (mainly oncologists)
  • Analyse immunohistochemistry and next generation sequencing results
  • Be responsible within a team of the OncoDNA’s database
  • Assure the link between subcontractors, production and customers

PhD (FRIA fellowship)

Department of Neuroscience, University of Mons, Mons, Belgium
2015 to 2019
  • PhD thesis : « Effects of anti-GAD65 antibodies on the inflammatory state and the synaptic plasticity of the hippocampus in an in vivo model »
  • DEVELOPMENT OF AN IN VIVO MODEL OF CEREBRAL INFUSION OF ANTI-GAD65 ANTIBODIES AND CHARACTERIZATION OF THIS MODEL : more than 140 stereotactic surgeries (mice)
  • EX VIVO ELECTROPHYSIOLOGICAL MEASURES OF THE LTP : approximatively 120
  • IMMUNOHISTOCHEMISTRY AND HISTOLOGICAL COLORATIONS : 15 different stainings
  • DETECTION OF CYTOKINES BY ELISA: 3 different kits
  • QUANTIFICATION OF THE EXPRESSION OF DIFFERENT RECEPTORS AND SYNAPTIC PROTEINS BY WESTERN-BLOTTING: 15 different antibodies
  • BEHAVIORAL TESTS : Morris Water Maze (40 mice) and Contextual Fear Conditioning (30 mice)
  • PATCH-CLAMP RECORDINGS : learning with a collaborator
  • GENOTYPING OF TRANSGENIC MICE : approximatively 150 mice

Master thesis

Department of Neuroscience, University of Mons, Mons, Belgium
February 2014 to December 2014
Student Project
Mons
  • Experimental autoimmune encephalomyelitis : Study of the neuroinflammation and its impact on the synaptic plasticity of the hippocampus
  • EX VIVO ELECTROPHYSIOLOGICAL MEASURES OF THE LTP : around 60
  • IMMUNOHISTOCHEMISTRY AND HISTOLOGICAL COLORATIONS : 4 different stainings
  • BEHAVIORAL TESTS : Contextual Fear Conditioning (24 mice)

Internship in a Neuroscience Research Center (during the master)

Neuroscience Research Center of Lyon – Reference Center for Paraneoplastic Neurological Syndromes - University of Lyon - Neurological Hospital of Lyon
February 2015 to May 2015
Internship
Lyon
France
  • Study of antigenic targets involved in limbic encephalitis
  • CELL CULTURE : more than 15
  • MANIPULATION OF HUMAN SAMPLES : approximatively 100
  • CELL-BASED ASSAYS : more than 20

Internships in Hospitals (during the bachelor)

CHU Charleroi - Centre de Santé des Fagnes
May 2011 to June 2011
Internship
Belgium
  • Department of Hematology, Pathological Anatomy, Bacteriology and Clinical chemistry
  • TESTS, CLINICAL ANALYSES AND INTERPRETATIONS

Internship in an External University (during the bachelor)

Laboratory of Experimental Neurology, Université Libre de Bruxelles
January 2011 to April 2011
Internship
Bruxelles
Belgium
  • Generation of neural stem cells from human induced pluripotent stem cells
  • CELL CULTURE of human IPSC
  • IMMUNOCYTOCHEMISTRY TECHNIQUES: 5 different stainings
Education

Doctoral Formation in Biomedical Sciences (PhD)

University of Mons, Mons, Belgium

Since 2015
Department of Neuroscience

Master in Biomedical Sciences (Magna Cum Laude)

University of Mons, Mons, Belgium

2011 to 2015
With Preparatory years in Biomedical Sciences (Cum Laude)

Bachelor in Medical Biology (Cum Laude)

Haute Ecole Provinciale de Hainaut-Condorcet, Montignies-Sur-Sambre, Belgium

2008 to 2011
Skills

Scientific

  • Electrophysiology (LTP)
    Advanced
  • Cell culture and iPSC culture
    Advanced
  • Stereotactic surgery
    Advanced
  • Cell-based assays
    Advanced
  • Behavioral tests with mice
    Advanced
  • Genotyping
    Advanced
  • Western-blotting
    Advanced
  • ELISA
    Advanced
  • Immuno-histo and cyto-chemistry
    Advanced
  • Immunofluorescence microscopy
    Advanced
  • Confocal microscopy
    Good
  • Next generation sequencing
    Good
  • FELASA C Certificate
    Expert
  • GMP/GLP
    Advanced

Soft Skills

  • Teamwork
    Expert
  • Creative problem solving
    Advanced
  • Communication
    Advanced
  • Innovation management
    Advanced

Publications or Communications in English

  • Redaction of the chapter "MULTI-ACTIONS OF MICROGLIA" in the book "Neuroimmune diseases" (July 2019)
  • Oral communication during the PhD day (2019)
  • Poster presentation (5) in international and national congressess (2016-2019)
  • Participation to the "Catania International Summer School of Neuroscience" and interactions with numerous scientists - Animal models of human brain diseases (2017)
  • Native language : French
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